To make mycelium liquid fermentation, breathable lid jars were used. The jars were filled with liquid malt medium.and sterilized. Each jar was inoculated with 1cm² of Reishi (Ganoderma lucidum) mycelium grown on agar. Reishi mycelium was used as it is the most widely used strain for mycelium leather production for its resistance and thickness.
Mycelium first started growing submerged and fluffy and then colonized the entiere top of the liquid where the oxygen was more abundant, leading to a liquid state surface fermentation.